Component analysis and Identification of the n-Hexane Fraction in Ethanolic Extract of Adlay Bran

• Main Authors: Shin-Chiai Chen, 陳欣喬
• Other Authors: 江文章
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/92012623534584563020

碩士 === 臺灣大學 === 食品科技研究所 === 98 === Adlay (Coix lachrymal-jobi L. var. ma-yuen Stapf) is an one year grass crop that has long been used as traditional Chinese medicine and a nourishing food. Recent studies have shown the effects of adlay are benefit to treat tumor and inflammation. Furthermore, the dehulled adlay has higher activity than polished adlay, especially in the adlay bran. In China, adlay seed was extracted with supercritical CO2 to get the oily extract that is formulated into an emulsion for injection (Kanglaite injection (KLT)). KLT injection showed the antitumor proliferation and life extension in animal tumor models. It demonstrated the potential for antitumor and anti-cachexia activity of the low polarity fraction of adlay seed. The adlay bran contain about 25% crude fat (Wt%), however, there are less research results shown the characteristic of the low polarity fraction of adlay seed or bran. Therefore the aim of this study is to analyze the composition in the n-hexane fraction of adlay bran ethanolic extract by gas chromatography / mass spectrometer (GC / MS).And column chromatography were employed to afford pure compounds. The study results revealed that there are 32 components identified in the GC / MS analysis, including 7 phytosterol; 3 monoglyceride; 7 free fatty acid & its ester; 1 amide; 1 alcohol; 1 alkene; 7 aldehyde; 5 other species of components. In addition, 3 pure compounds are isolated and identified: stigmasterol, beta-sitosterol, 3-O-feruloyl-5beta-sitostan-3-ol, and 1, 2-, 1, 3-diglyceride mixture. According to the literature review, the possible active components in the n-hexane fraction of adlay bran ethanolic extractare are phytosterol, monoglyceride, and fatty acid amide.