Utilizing intravital multi-photon microscopy to monitor the effects of fatty liver disease on hepatobiliary metabolism

• Main Authors: Shu-Yen Lo, 羅書硯
• Other Authors: 董成淵
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/4u5n9c

碩士 === 國立臺灣大學 === 物理研究所 === 98 === Liver disease represent a major health problem worldwide. Fatty liver is a common liver disease which can progress to non-alcoholic steatohepatitis (NASH), fibrosis, cirrhosis. In this thesis, in order to elucidate the effects of fatty liver on hepatobiliary metabolism, we combined multi-photon microscopy with a home-built intravital hepatic imaging chamber (IHIC) to image and quantify the metabolic capacity of liver in methionine- and choline-deficient (MCD) dietary animal model. Mice were imaged at 2, 4, 6, and 8 weeks after they were first fed with MCD. Non-fluorescent 6-carboxyfluorescein diacetate (6-CFDA) which is hydrolyzed by the hepatocytes into fluorescent 6-carboxyfluorescein (6-CF) and excreted into the bile canaculi by the multidrug resistant proteins (mrps) is then used as the molecular probe in tracing the effects of fatty liver on hepatobiliary metabolism. Our data showed that the metabolic function of liver in fatty liver diseased mice is impaired in comparison with that of normal mice.