| Summary: | 碩士 === 國立臺東大學 === 生命科學系碩士班 === 98 === Ferredoxin is one of the electron transferring protein. It exists in differs space of
plant and regulates many fundamental-metabolism. Recently, the invertation of revaled
that the storage tissue of plant contains high quantity of Non-photosynthetic type Fd
(NT-Fd).However the role for fundamental-metabolism of NT-Fd was unclear. In this
study the role of NT-Fd in growth of Arabidopsis thaliana cv. columbia was investigated
by CaMV 35S promoter was used to overexpression NT-Fd of Arabidopsis AtFd3 in
transgenic lines. The result indicates AtFd3 transgenic Arabidopsis line was
overexpressed AtFd3 two to five folds The growth of the wild-type and atfd3 transgenic
Arabidopsis was same in both low glucose content (1% glucoses) and high glucose
content (3% glucoses) environment. However AtFd3 transgenic Arabidopsis were
delayed entering the reproduction stage in the low glucose content environment. By
contrast, the atfd3 transgenic Arabidopsis was increased entering senescence stage in
pot. The starch content of root tissue of atfd3 transgenic Arabidopsis was increased in
low glucose content environment, but reduced in high glucose content environment. In
the leaves tissue, it was increased. On the other hand, the starch content of leaves tissue
was reduced at 30 day, but increased at 90 day in pot. The geotropism behavior of atfd3
transgenic Arabidopsis was increased at pH 5 environment. The phenomenon was
performed in the seeding cut. Finally, The auxin and cytokinin sensitivity of atfd3
transgenic Arabidopsis was increased. Therefore overexpression of AtFd3 in atfd3
transgenic Arabidopsis was increased that NAA or kinetin inhibit ability of the growing
root. According to above results, the overexpression of NT-Fd, AtFd3, can influence that
growth and geotropism behavior of plant result from the enhancement of auxin and
cytokinin sensitivity and the content of starch.
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