| Summary: | 碩士 === 國立臺灣海洋大學 === 電機工程學系 === 98 === This study utilizes micro-electro-mechanical process technology to fabricate a micro-fluidic chip. Sputtering is used to deposit a roughly 150nm thick layer of platinum on a glass plate, which is used as the electrode. Finally, the PDMS micro-fluidic channel and the glass plate with a platinum layer are joined together to produce a DNA test micro-fluidic chip that has a metallic layer and hollow micro-fluidic channel. For biological samples, Edwardsiella tarda and Aeromonas Hydrophila are used as standard strains, and tested for DNA hybridization using magnetic bead-based probes, conducting impedance tests at the same time.
The experiment uses U-101 magnetic beads, in the DNA hybridization sample test; DNA10670 and U-101 + ET996 are complementary and can be hybridized, DNA13881 and U-101 + ET996 are not complementary and cannot be hybridized. Tests on the samples have found that the impedance of different concentrations of U-101 + ET996 + DNA10670 is in the range 957.28Ω ~ 1.2537KΩ, the impedance of different concentrations of U-101 + ET996 + DNA13881 is in the range 835.78Ω ~ 863.27Ω. Under the same concentration, impedance is significantly different when there is and is not hybridization; this difference can be used as a basis for determining whether or not there is DNA hybridization.
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