The Effect of Methylmercury and Inorganic Mercury On Tissue Accumulation and Elimination in Orange-spotted Grouper (Epinepheluscoioides) Following Oral Administration

• Main Authors: Yao-Ren Hsu, 許堯仁
• Other Authors: Fan-Hua Nan
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/61452709183275635683

碩士 === 國立臺灣海洋大學 === 水產養殖學系 === 98 === The objective of this study is to investigate the accumulation and elimination of methylmercury and inorganic mercury in different tissues including feces, muscle, liver, skin, stomach, intestine, gill filament, kidney, spleen, blood, brain and heart in Orange-spotted Grouper (Epinephelus coioides) after oral administration at concentration of 1 mg/kg and 5 mg/kg for methylmercury and inorganic mercury. The results showed that in inorganic mercury treatment group, except for intestine, kidney and gill filament tissues which showed significantly higer total mercury concentrations, there is no significant difference between the concentrations of total mercury in other tissues and the control group. In addition considerable amount of total mercury and some methylmercury were detected in feces. In methylmercury treatment group, the concentrations of total mercury and methylmercury in tissues were significantly higher than that in the control group; and total mercury and methylmercury can be detected in the feces. For elimination study, methylmercury that accumulated in muscle and brain can hardly be eliminated. However, the concentrations of methylmercury in other tissues such as kidney, liver and gill filament decreased rapidly 7 days post oral administration. In 5 mg/kg methylmercury treatment group, methylmercury was accumulated in tissues in the order of kidney (1856.67 μg/kg)> intestine (1383.33 μg/kg)> liver (1176.67 μg/kg)> heart (883.00μg/kg)> stomach (723.33 μg/kg)> spleen (685.33 μg/kg)> brain (528.00 μg/kg)> muscle (392.67 μg/kg)> gill filament (367.00 μg/kg)> skin (194.67 μg/kg) referring to its concentration. After 7 days oral administration of 1 mg/kg and 5 mg/kg methylmercury, the absorption of methylmercury in tissues were approximately 50 % and 30 %, respectively. In the methylmercury treatment group, the concentrations of total mercury and methylmercury in muscle, liver, stomach and skin were significantly higher than that in control group and inorganic mercury treatment group. The percentages of methylmercury in the total mercury for muscle, liver, stomach, intestine and skin were found to be in the range of 63.88 % - 89.84 %, 63.97 % - 90.27 %, 7.62 % - 88.63 %, 1.71 % - 95.77 %, 27.46 % - 97.72 %, respectively.