| Summary: | 碩士 === 國立清華大學 === 奈米工程與微系統研究所 === 98 === Almost all organs or tissues are not able to regenerate by themselves again; hence, the only way we can repair our tissues or organs is to utilize transplant techniques. However, today we face some serious problem derived from transplantation, such as, the insufficient source from organ donation and immune system rejection. Recent years, there is a great development of tissue engineering. In today's digestive tract disease, because of removing their small intestine, patients who endowed with short bowel syndrome are suffered from absorption of nutrients, thus threatening people's lives. By adding new small intestine in vitro as well as transplanting it into the human body, intestinal tissue engineering attempts to save patients who suffered by short bowel syndrome. In tissue engineering, if we can provide a scaffold that is with the same shape as well as size of tissues, we can take advantage of this scaffold to regenerate new tissues and thereby repair human small intestine. Taken together, this study not only aims to produce a biomimetic structure scaffold of intestinal villi, but also to provide a better atmosphere for the growth of intestinal cells as well. Aforementioned technique can increase surface area of the structure as well as improve the original problem of scaffold.
In order to produce biomimetic scaffold structure of intestinal villi, our laboratory developed backside exposure technology. In the photolithography process, optical diffraction and material absorption effect exerts a significant influence on the microstructure shape. Hence, this thesis attempts to explore the diffraction, absorption and photoresist threshold of dose on the basis of Matlab simulation of optical diffraction.
This thesis utilize SU-8 photoresist, molding technology and PLA materials to produce micro-structure, sequentially immersing the the PLA structure into acetone diluted among the top and thereby shifting the geometry of the structure that enables the structure more close to the shape of the small intestine villi. Through our experiment, we acquire a set of best parameters to produce the biomimetic scaffold structure of intestinal villi and cultured in the scaffold structure of intestinal cells into tissues. As a consequence, we successfully attached on the 3D structure to the tissues, by observing the growth of cell growth on the basis of SEM and Confocal.
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