利用神經基板裝置研究中樞神經細胞軸突受損後軸突退化與再生的變化

• Main Authors: Chung, Hui-Wen, 鍾惠雯
• Other Authors: Chang, Yen-Chung
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/67501057136866323439

碩士 === 國立清華大學 === 分子醫學研究所 === 98 === Nerve regeneration is an important issue in neuromedical research. Accumulating evidence has indicated that the axons in adult mammalian brain and spinal cord do not regenerate after injury. On the other hand, in the peripheral nervous system, nerves exhibit remarkable capability of regeneration after injury. It remains rather difficult to cure patients suffering from many diseases wherein the axons in the central nervous systems have been injured. Recent discoveries have also indicated that axonal degeneration occurring in diseased nervous systems would eventually cause neuronal death. Thus, studying the mechanism of axonal degeneration is considered ancillary of studying the mechanisms underlying neuronal regeneration. More recently, our laboratory develop has developed a neuronal chip device. On the chip surface, axons of cultured rat hippocampal neurons are guided along poly-L-lysine-coated fine lines to grow in areas distinct from where their cell bodies reside. Therefore, we can observe the morphological change and protein expression on cell bodies and axons separately after axotomy. This study consisted of three parts. The first part consisted of the observations of the morphological changes of cell bodies and axons at different times after axotomy. The second part consisted of studies of the effects of different extrinsic proteins on the degeneration and re-growth of axons after axotomy. The third part focused on analyzing local protein expression in the axon by using Click Chemistry and confocal microscopy. The results indicated that the majority of neurons on the chip could survive axotomy and that, after axotomy and subsequent degeneration of distal axon fragments, new axons could re-grow along the poly-L-lysine-coated fine lines into the areas on the chip designated to be filled by axons. A number of factors have been known to affect the process of axonal degeneration and regeneration. Here, the study has indicated that chip device developed by our laboratory is a useful tool for studying the mechanisms underlying axonal degeneration and regeneration. The results may help the development of new therapeutic strategies for axonal regeneration in the future.