Immunogenic Analysis of the Recombinant Outer Membrane Lipoprotein Lpp38 of Mannheimia haemolytica

• Main Authors: Nanthiya Sangplao, 南西亞
• Other Authors: Chun-Yen, Chu
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/62339988255201597635

博士 === 國立屏東科技大學 === 熱帶農業暨國際合作系所 === 98 === Pneumonic pasteurellosis, caused by Mannheimia (Pasteurella) haemolytica (M. haemolytica), is one of the most economically important respiratory infectious diseases of ruminants with a wide prevalence throughout the world. Several studies implicated its outer membrane proteins (OMPs) as immunologically important surface antigens. The 38 kDa lipoprotein, Lpp38, is the immunogenic membrane protein of M. haemolytica represents in both outer and inner membranes. In previous studies, recombinant Lpp38 (rLpp38) from a reference strain (BCRC13946) was cloned, expressed, and used to enhance the efficacy of inactivated M. haemolytica vaccine in mice and goat models. In this study, rLpp38 of M. haemolytica from a local strain isolated from cattle (B2) in Taiwan was cloned and expressed. Mice immunized with rLpp38, either from B2 or BCRC13946, vaccine did not have significantly increased antibody titers when compared to the control group. However, mice vaccinated with rLpp38 (both B2 and BCRC13946) alone with adjuvant showed enhanced resistance against experimental challenge with live M. haemolytica. Vaccination of rLpp38 (B2) in goat resulted in an increased antibody titer against M. haemolytica whole cell antigen. The antibody titer reached the peak at week 3 and declined gradually after that. The results from this study indicate that more suitable animal was required for experimental challenge with live M. haemolytica.