| Summary: | 碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 98 === Mannheimia haemolytica is a member of the Pasteurellaceae family, which is the major cause of acute fibrinous pneumonia when ruminant face the situation of stress. The mortality rate of pneumonic pasteurellosis is 30% approximately. It is urgency to develop new vaccine to prevent M. haemolytica infection and reduce the economical loss. The previous studies indicated that the outer membrane protein PlpE (Pasteurella haemolytica lipoprotein E) is the most important antigen and the leukotoxin (Lkt) play a major role in lung injury. In this study, we cloned the gene of lkt and plpe from different strains to express recombinant proteins (rLkt’C, rPlpE) by the prokaryotic system and sequences pair-wise alignment. The rLkt’C and rPlpE were recognized by sera from immunized goat. The vaccines made of inactivated M. haemolytica and recombinant protein (rPlpE, rLkt’C) with w/o/w adjuvant. In mice study, the antibody titer and T cell proliferation index of immunized group was significantly higher than control group
(p<0.05), and the survival rate of protection efficacy was 80%. In goat study, both inactivated M. haemolytica and rLkt’C group show significant higher ELISA antibody titers than control group at the four weeks post-vaccination (p<0.05). In summary, these expressed proteins may be used as recombinant protein vaccine candidate against M. haemolytica.
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