Inhibitory effect of Psidium guajava L. and Morus alba L. leaves extracts on alpha-glucosidase activity and postprandial blood glucose

• Main Authors: Yen-Ping Hsu, 許燕萍
• Other Authors: Pao-Chuan Hsieh
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/16236693602712548024

碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Diabetes is one of leading causes of death, according to the statistics by Department of Health , Executive Yuan of Taiwan. In modern medicine, diabetes could be controlled by various medication. However, drugs always have undesirable side effects. Therefore, it becomes the goal to find the plant content which can decrease glucose concentration of the blood, reduce the incidence of complication, and prevent the side effects. Alpha-Glucosidase inhibitors (AGIs) are the effective glucose-lowering medicines, and is a key enzyme of carbohydrates digestion. Inhibition of α-glucosidase leads to a delayed and reduced rise in post-prandial blood glucose levels. The purpose of this study is to evaluate inhibitory effect of mixture extracts of P. guajava L. and M. alba L. leaves on α-glucosidase activity and postprandial hyperglycemia in normal and diabetic rats. The inhibition of α-glucosidase activity was assayed in vitro. The IC50 values of Psidium guajava L. and Morus alba L. were 2.25 mg/mL and 0.1 mg/mL, espectively. The IC50 value of commercial anti-hyperglycemic agent (Glucobay) was 6.41 mg/mL. The IC50 value of mixture extracts of P. guajava L. and M. alba L. was 0.07 mg/mL. In cytotoxicity test, murine embryonic liver cell line (BNL CL.2) which were treated with various concentration and various time of mixture extracts, the survival percentages and the shape were no significant effect. In vivo, after feeding 8 weeks with mixture extracts, the results of oral sucrose challenge test showed that the extracts can reduce sucrose hydrolysis to monosaccharide, and delay the absorption of glucose, leading to the suppression of meal-induced increase of glucose. The mixture of plants can decrease in triglyceride, glutamic-oxalocetic transaminase(GOT), glutamic- pyruvic transaminase(GPT) concentration in plasma, and increase HDL concentration. In histopathology assay, the DCL, DCM, DCH, DCG of diabetic-induced groups were observed slight pathology less than diabetic control(DC) group.