Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid and strong lemon odor. The mixture of compounds are double bond isomers. The E-isomer is known as geranial or citral A. The Z-isomer is known as neral or citral B. There are several an...
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ndltd-TW-098NPUS52530282016-12-22T04:18:20Z http://ndltd.ncl.edu.tw/handle/77900668101932339341 Detoxifying enzymes induction effect of citral in Hep G2/C3A cell 檸檬醛在肝癌細胞Hep G2/C3A 中誘發解毒酵素調控機制 Yun-Ling Tai 戴韻伶 碩士 國立屏東科技大學 食品科學系所 98 Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid and strong lemon odor. The mixture of compounds are double bond isomers. The E-isomer is known as geranial or citral A. The Z-isomer is known as neral or citral B. There are several antioxidant-response element (ARE)-regulated gene products such as glutathione S-transferase, NAD(P)H:quinine oxidoreductase 1, glutamate cysteine ligase, and hemeoxygenase-1 are known to mediate detoxification and to exert antioxidant functions thereby protecting cells from genotoxic damage. The transcription of ARE-driven genes is regulated, at least in part, by nuclear transcription factor erythroid 2p45 (NF-E2)-related factor 2(Nrf2), which is sequestered in cytoplasm by Kelch-like ECH-associated protein 1 (Keap1). This experiment will investigate the effects of citral on the glutathione (GSH) production, phase II detoxifying enzymes protein expression, Nrf2 protein levels and translocation and it regulatory pathways. In the results, concentration of citral under 100 μM showed no toxic effect on Hep G2/C3A cell lines. We found that the pathway of citral activation of Nrf2 through IV phosphorylation by Akt, JNK and ERK. We also found that increase in Nrf2 accumulation in the nuclear fraction, induce phase II detoxify enzyme, including GCLC, GST and HO-1 and induction of GSH synthesis. Tzou-Chi Huang 黃卓治 2010 學位論文 ; thesis 66 zh-TW |
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碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid
and strong lemon odor. The mixture of compounds are double bond isomers.
The E-isomer is known as geranial or citral A. The Z-isomer is known as
neral or citral B. There are several antioxidant-response element
(ARE)-regulated gene products such as glutathione S-transferase,
NAD(P)H:quinine oxidoreductase 1, glutamate cysteine ligase, and
hemeoxygenase-1 are known to mediate detoxification and to exert
antioxidant functions thereby protecting cells from genotoxic damage. The
transcription of ARE-driven genes is regulated, at least in part, by nuclear
transcription factor erythroid 2p45 (NF-E2)-related factor 2(Nrf2), which is
sequestered in cytoplasm by Kelch-like ECH-associated protein 1 (Keap1).
This experiment will investigate the effects of citral on the glutathione (GSH)
production, phase II detoxifying enzymes protein expression, Nrf2 protein
levels and translocation and it regulatory pathways. In the results,
concentration of citral under 100 μM showed no toxic effect on Hep G2/C3A
cell lines. We found that the pathway of citral activation of Nrf2 through
IV
phosphorylation by Akt, JNK and ERK. We also found that increase in Nrf2
accumulation in the nuclear fraction, induce phase II detoxify enzyme,
including GCLC, GST and HO-1 and induction of GSH synthesis.
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author2 |
Tzou-Chi Huang |
author_facet |
Tzou-Chi Huang Yun-Ling Tai 戴韻伶 |
author |
Yun-Ling Tai 戴韻伶 |
spellingShingle |
Yun-Ling Tai 戴韻伶 Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
author_sort |
Yun-Ling Tai |
title |
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
title_short |
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
title_full |
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
title_fullStr |
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
title_full_unstemmed |
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell |
title_sort |
detoxifying enzymes induction effect of citral in hep g2/c3a cell |
publishDate |
2010 |
url |
http://ndltd.ncl.edu.tw/handle/77900668101932339341 |
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