Detoxifying enzymes induction effect of citral in Hep G2/C3A cell

碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid and strong lemon odor. The mixture of compounds are double bond isomers. The E-isomer is known as geranial or citral A. The Z-isomer is known as neral or citral B. There are several an...

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Main Authors: Yun-Ling Tai, 戴韻伶
Other Authors: Tzou-Chi Huang
Format: Others
Language:zh-TW
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/77900668101932339341
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spelling ndltd-TW-098NPUS52530282016-12-22T04:18:20Z http://ndltd.ncl.edu.tw/handle/77900668101932339341 Detoxifying enzymes induction effect of citral in Hep G2/C3A cell 檸檬醛在肝癌細胞Hep G2/C3A 中誘發解毒酵素調控機制 Yun-Ling Tai 戴韻伶 碩士 國立屏東科技大學 食品科學系所 98 Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid and strong lemon odor. The mixture of compounds are double bond isomers. The E-isomer is known as geranial or citral A. The Z-isomer is known as neral or citral B. There are several antioxidant-response element (ARE)-regulated gene products such as glutathione S-transferase, NAD(P)H:quinine oxidoreductase 1, glutamate cysteine ligase, and hemeoxygenase-1 are known to mediate detoxification and to exert antioxidant functions thereby protecting cells from genotoxic damage. The transcription of ARE-driven genes is regulated, at least in part, by nuclear transcription factor erythroid 2p45 (NF-E2)-related factor 2(Nrf2), which is sequestered in cytoplasm by Kelch-like ECH-associated protein 1 (Keap1). This experiment will investigate the effects of citral on the glutathione (GSH) production, phase II detoxifying enzymes protein expression, Nrf2 protein levels and translocation and it regulatory pathways. In the results, concentration of citral under 100 μM showed no toxic effect on Hep G2/C3A cell lines. We found that the pathway of citral activation of Nrf2 through IV phosphorylation by Akt, JNK and ERK. We also found that increase in Nrf2 accumulation in the nuclear fraction, induce phase II detoxify enzyme, including GCLC, GST and HO-1 and induction of GSH synthesis. Tzou-Chi Huang 黃卓治 2010 學位論文 ; thesis 66 zh-TW
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language zh-TW
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description 碩士 === 國立屏東科技大學 === 食品科學系所 === 98 === Citral (3,7-dimethyl-2,6-octadienal) that exist citrus fruit is yellow liquid and strong lemon odor. The mixture of compounds are double bond isomers. The E-isomer is known as geranial or citral A. The Z-isomer is known as neral or citral B. There are several antioxidant-response element (ARE)-regulated gene products such as glutathione S-transferase, NAD(P)H:quinine oxidoreductase 1, glutamate cysteine ligase, and hemeoxygenase-1 are known to mediate detoxification and to exert antioxidant functions thereby protecting cells from genotoxic damage. The transcription of ARE-driven genes is regulated, at least in part, by nuclear transcription factor erythroid 2p45 (NF-E2)-related factor 2(Nrf2), which is sequestered in cytoplasm by Kelch-like ECH-associated protein 1 (Keap1). This experiment will investigate the effects of citral on the glutathione (GSH) production, phase II detoxifying enzymes protein expression, Nrf2 protein levels and translocation and it regulatory pathways. In the results, concentration of citral under 100 μM showed no toxic effect on Hep G2/C3A cell lines. We found that the pathway of citral activation of Nrf2 through IV phosphorylation by Akt, JNK and ERK. We also found that increase in Nrf2 accumulation in the nuclear fraction, induce phase II detoxify enzyme, including GCLC, GST and HO-1 and induction of GSH synthesis.
author2 Tzou-Chi Huang
author_facet Tzou-Chi Huang
Yun-Ling Tai
戴韻伶
author Yun-Ling Tai
戴韻伶
spellingShingle Yun-Ling Tai
戴韻伶
Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
author_sort Yun-Ling Tai
title Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
title_short Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
title_full Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
title_fullStr Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
title_full_unstemmed Detoxifying enzymes induction effect of citral in Hep G2/C3A cell
title_sort detoxifying enzymes induction effect of citral in hep g2/c3a cell
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/77900668101932339341
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