| Summary: | 碩士 === 國立屏東科技大學 === 水產養殖系所 === 98 === Polysaccharide have widely applied as a dietary supplement in aquaculture. The objectives of this study were to elucidate the effects of dietary different treated Sargassum cristaefolium diets at 0 (control), hot-water extract of 0.25、0.5g kg-1, heat algae powder of 2.5g kg-1, or algae powder of 2.5g kg-1 to Litopenaeus vannamei on the growth, immune responses, disease resistance, and antistress. In the growth performance test, the percent weight gain and feed efficiency of shrimp fed extract of S. cristaefolium at 0.25、0.5g kg-1, and heat algae powder of 2.5g kg-1 were significantly higher than that of shrimp fed the control diet for 4 months. In the challenge test, the survival rate of the L. vannamei fed heat algae powder of S. cristaefolium at 2.5g kg-1 for 4 months, challenged with Vibrio alginilyticus, were significant higher than the shrimp fed the control diets. In the immune responses, the shrimp fed different treated S. cristaefolium diets for 0、1、2、4 months were used for evaluating the immune parameters. The results showed that phenoloxidase (PO) activity, respiratory bursts (O2-), superoxide dismutase (SOD) activity, phagocytic activity, and clearance efficiency of shrimp fed heat algae powder of S. cristaefolium at 2.5g kg-1 were significant higher than those of shrimp fed the control diets for 4 months. The immune responses of the shrimp stressed in high temperature at 34℃ for 0、2、4、16 hours after feeding different treated S. cristaefolium diets for 4 months were evaluated. The results showed the immune levels were maintained under stress after shrimp fed heat treated S. cristaefolium diets. Therefore, it is concluded that dietary administration of heat algae powder of S. cristaefolium at 2.5g kg-1 for 4 months to L. vannamei induced growth performances, immune ability, antistress, and disease resistance to V. alginilyticus.
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