| Summary: | 碩士 === 國立澎湖科技大學 === 海洋創意產業研究所 === 98 === Abstract
The vitellogenin synthesis and accumulation in oocytes is the first key step of reproduction for oviparous animals. The oviparous grass shrimp Penaeus monodon, in which the hepatopancreas is the extraovarian site of vitellogenesis, is one of the most important crustacean species for aquaculture industry. Biogenic amines which are widely distributed over the animal bodies including most parts of brain act as neurotransmitters. It was elucidated that some of biogenic amines correlated closely with crustacean reproduction. In this study we revealed that administration of one biogenic amine or its precursor does induce and stimulate the ovarian maturation even to spawning in pond maricultural grass shrimp. The maturation can also be induced in these shrimps by unilateral eyestalk ablation. Vitellin in grass shrimp is composed of seven major components revealed by 7%T SDS-PAGE, and the molecular weights of these components are then estimated as 78.8, 86.1, 97.1, 112.7, 123.3, 168.6 and 184.4 kDa respectively. Rabbit antisera against two components of vitellin, 112.7 and 123.3 kDa, are employed in the ELISA procedure for titration of vitellogenin concentration in shrimp hemolymph. The average of hemolymph vitellogenin concentrations are estimated as 3.006mg/ml in the female shrimps which are unilateral eyestalk ablated, and 1.061mg/ml in the female shrimps received biogenic amine administration respectively.
Keywords: Penaeus monodon, vitellogenin, biogenic amine, reproduction
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