Usage of umbilical cord mesenchymal stem cells transduced with DcR3 by lentivirus for the treatment type1 diabetes mellitus

• Main Authors: Weng,Wei-Hsiung, 翁偉雄
• Other Authors: Jia-Fwu Shyu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/41601786604889385706

碩士 === 國防醫學院 === 生物及解剖學研究所 === 98 === Type 1 diabetes is caused by T cell mediated autoimmune destruction of pancreatic β-cells. Recently stem cell therapeutic approach has been implicated for the treatment of diabetes. Mesenchymal stem cells (MSCs) possess multilineage property making them useful for a number of potential therapeutic applications. Decoy Receptor 3 (DcR3), belongs to tumor necrosis factor receptor super family, is a decoy receptor for Fas ligand (FasL). DcR3 binds to FasL and suppresses immune function of T-cells. The aim of this study was to analyze the diabetes therapeutic potential of the MSCs transduced by DcR3-GFP lentivral vector in the Non-obese diabetes (NOD) mice. MSCs isolated from human Wharton`s jelly and transduced GFP and DcR3-GFP gene by lentivirus. These cells were injected into retro-orbital venous sinus in NOD mice. Significant decreases of blood glucose, higher survival rate and increased tolerance of the blood glucose change after intraperitoneal glucose injection were found in NOD mice injected with both of MSCs and lentivirus-infected MSCs compared to those with PBS normal control. Colocalization of human C-peptide and GFP in the pancreas of NOD mice transplanted with lentivirus-infected MSCs, Human C-peptide could be detected in serum from NOD mice transplanted by Lentivirus-infected MSCs and significant high of human C-peptide level in transplant DcR3-GFP infected MSCs group compare with control group . In conclusion, MSCs from Wharton`s Jelly are potential useful source for cell therapy of diabetes and could be track by the GFP.