SHP-2 Promotes Neural Progenitor Cell Migration through Focal SHP-2 Promotes Neural Progenitor Cell Migration through Focal Adhesions

• Main Authors: Chu, Chun-Ming, 朱俊銘
• Other Authors: Ma, Kuo-Hsing
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/87781021675766196934

碩士 === 國防醫學院 === 生物及解剖學研究所 === 98 === SHP-2 is tyrosine phosphatase, and widely exists in different cells. SHP-2 regulates growth, mitosis, motility and differentiation by growth factors or cytokines. SHP-2 exists in neural progenitor cell, but there are only little studies about SHP-2 function and pathway. Neurospheres are source of neural progenitor cells, and they can differentiate into different neural cells. The previous study showed phospho-SHP-2 expressed at leading edge of neural progenitor cell, and colocalized with phospho-paxillin and phospho-FAK at focal adhesions. Neurosphere-derived neural progenitor cells, neurons and astrocytes are with same focal adhesion staining pattern. This study explored migration signal mechanism of SHP-2. This study showed when neurosphere cell attached and migrated, phospho-paxillin, phospho-FAK and phospho-SHP-2 were increased. This study indicated SHP-2 would be recruited to focal adhesion, and then would be activated, and regulated assembly of focal adhesion. To elucidate SHP-2 signal pathway in regulating migration, this study used protein tyrosine phosphatase inhibitor IV and siRNA to reduce SHP-2 activity and expression. Those results showed distance of neurosphere cell migration were reduced, and morphology of astrocyte was changed, and phospho-paxillin and phospho-FAK were simultaneous reduced. In the conclusion, when neural progenitor cell attaches extracellular matrix, SHP-2 will gather at focal adhesion, and then be activated to regulate cytoskeletal reorganization and assembly of focal adhesion, and thereby regulates neural progenitor-derived cell migration and morphogenesis.