| Summary: | 碩士 === 國防醫學院 === 生物化學研究所 === 98 === Ribosomal protein S19 is a component of 40S Ribosome subunit，previous study found Ribosomal protein S19 except to participate in ribosome synthesis, also have the functions of gene translation regulations. In this study, we used Biochip assay screening cDNA from colon cancer patient stool which detected the RPS19 gene overexpressed in primary human colon cancer patients. To understand the rule of RPS19, Hct116 p53+/+ and Hct116 p53-/- cells infected with lentivirus expressing RPS19 siRNA to established sh-Luc and sh-RPS19 cell lines. The effects of RPS19 on colon cancer cell growth and cell cycle were evaluated with MTT assay and Flow cytometry, the data showed decreased RPS19 gene caused cells grew much slower and induced G0/G1 arrest, we also treated Oxaliplatin, a chemotherapeutic drug, caused p21 and r-H2AX induction in sh-RPS19 cell line, and furthermore we speculated that decreased RPS19 expression may blocks 26S proteasome pathway than accumulated p21 protein. In conclusion, the study suggested decrease RPS19 gene expression may inhibited cancer cell growth and exhibited synergistic effect on Oxaliplatin treatment. In recent years there has been found many research of RPS19 protein structures and interaction proteins, they also indicated that RPS19 may be an important role in translation regulation. The molecular mechanisms of RPS19 in the tumorigenesis still have more study to investegated.
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