Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene

碩士 === 國立東華大學 === 海洋生物多樣性及演化研究所 === 98 === Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environments. Toxicity of PAHs has been extensively studied in many vertebrate and invertebrate species but not in corals. In this study, we employed a “nubbin assay” on 452 f...

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Main Authors: Ying-Ying Li, 李盈穎
Other Authors: Te-Hao Chen
Format: Others
Language:zh-TW
Published: 2010
Online Access:http://ndltd.ncl.edu.tw/handle/86190753457608629872
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spelling ndltd-TW-098NDHU52700162016-04-22T04:23:11Z http://ndltd.ncl.edu.tw/handle/86190753457608629872 Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene 多環芳香烴benzo(a)pyrene對萼柱珊瑚(Stylophorapistillata)之生理及基因表現之影響 Ying-Ying Li 李盈穎 碩士 國立東華大學 海洋生物多樣性及演化研究所 98 Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environments. Toxicity of PAHs has been extensively studied in many vertebrate and invertebrate species but not in corals. In this study, we employed a “nubbin assay” on 452 fragments of the coral Stylophora pistillata to characterize the toxic effects of benzo[a]pyrene [ B(a)P ] on corals at organismal, physiological, and molecular levels. Stylophora pistillata nubbins (5-10 polyps each) were exposed to B(a)P (0, 8, 49, and 180 μg/L in the control, low, medium, and high concentration groups, respectively) for 96 hr. Coral photosynthesis efficiency was measured with a DIVING-PAM Underwater Fluorometer, survival was monitored, and solutions were changed every 24 hr during the exposure period. At 96 hr of exposure, part of the nubbins were processed for total RNA extraction for gene expression analysis, and the other nubbins were moved into clean seawater for recovery for another 20 days. From 48 hr of exposure, photosynthesis efficiency of the high concentration group was significantly decreased. From 72 hr of exposure, significant decrease of photosynthesis efficiency was also observed in the medium concentration group. At 96 hr of exposure, survival rates in the control, low, medium, and high groups were 99, 100, 76, and 0%, respectively. For the survived nubbins moved into clean seawater, after the 20 days of recovering period, the survival rates in the control, low, and medium groups were 90%, 81%, and 82%, respectively. We used the quantitative PCR (qPCR) technique to analyze expression level of four biomarker genes, including CYP3, Mn SOD, PHGPx and HSP70, in the control and low concentration groups. In the low concentration group, expression of CYP3 was significantly induced, but that of Mn SOD, PHGPx and HSP70 was not affected. In conclusion, higher levels of B(a)P exposure decreased coral photosynthesis efficiency and survival. While low level B(a)P exposure did not cause observable effect on survival and photosynthesis efficiency, it did induce expression of a phase I detoxification enzyme gene CYP3, suggesting future application of this gene as a biomarker for PAH exposure in corals. Our results showed that acute B(a)P exposure may cause toxic effects on corals at multiple biological levels. Te-Hao Chen 陳德豪 2010/07/ 學位論文 ; thesis 59 zh-TW
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language zh-TW
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sources NDLTD
description 碩士 === 國立東華大學 === 海洋生物多樣性及演化研究所 === 98 === Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environments. Toxicity of PAHs has been extensively studied in many vertebrate and invertebrate species but not in corals. In this study, we employed a “nubbin assay” on 452 fragments of the coral Stylophora pistillata to characterize the toxic effects of benzo[a]pyrene [ B(a)P ] on corals at organismal, physiological, and molecular levels. Stylophora pistillata nubbins (5-10 polyps each) were exposed to B(a)P (0, 8, 49, and 180 μg/L in the control, low, medium, and high concentration groups, respectively) for 96 hr. Coral photosynthesis efficiency was measured with a DIVING-PAM Underwater Fluorometer, survival was monitored, and solutions were changed every 24 hr during the exposure period. At 96 hr of exposure, part of the nubbins were processed for total RNA extraction for gene expression analysis, and the other nubbins were moved into clean seawater for recovery for another 20 days. From 48 hr of exposure, photosynthesis efficiency of the high concentration group was significantly decreased. From 72 hr of exposure, significant decrease of photosynthesis efficiency was also observed in the medium concentration group. At 96 hr of exposure, survival rates in the control, low, medium, and high groups were 99, 100, 76, and 0%, respectively. For the survived nubbins moved into clean seawater, after the 20 days of recovering period, the survival rates in the control, low, and medium groups were 90%, 81%, and 82%, respectively. We used the quantitative PCR (qPCR) technique to analyze expression level of four biomarker genes, including CYP3, Mn SOD, PHGPx and HSP70, in the control and low concentration groups. In the low concentration group, expression of CYP3 was significantly induced, but that of Mn SOD, PHGPx and HSP70 was not affected. In conclusion, higher levels of B(a)P exposure decreased coral photosynthesis efficiency and survival. While low level B(a)P exposure did not cause observable effect on survival and photosynthesis efficiency, it did induce expression of a phase I detoxification enzyme gene CYP3, suggesting future application of this gene as a biomarker for PAH exposure in corals. Our results showed that acute B(a)P exposure may cause toxic effects on corals at multiple biological levels.
author2 Te-Hao Chen
author_facet Te-Hao Chen
Ying-Ying Li
李盈穎
author Ying-Ying Li
李盈穎
spellingShingle Ying-Ying Li
李盈穎
Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
author_sort Ying-Ying Li
title Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
title_short Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
title_full Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
title_fullStr Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
title_full_unstemmed Organismal, physiological and molecular responses of the coral Stylophora pistillata exposed to benzo[a]pyrene
title_sort organismal, physiological and molecular responses of the coral stylophora pistillata exposed to benzo[a]pyrene
publishDate 2010
url http://ndltd.ncl.edu.tw/handle/86190753457608629872
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