Distribution and Analysis of Enterotoxins, Antibiotic Resistant Genes of Staphylococcus spp. Isolated from Dairy Goat Farms

• Main Author: 李研懷
• Other Authors: 蘇耀期
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/11095668772082708265

碩士 === 國立嘉義大學 === 獸醫學系研究所 === 98 === Staphylococcus spp. can survive in broad environments, including food, dirt, appliance, and human or animals body skin and duct. Staphylococcal enterotoxins are the main virulence factor to human. In addition, misuse of antimicrobial drugs increases the emergence of multi-drug resistant staphylococci, which become public health problem. 98 staphylococci (27 S. aureus, 31 S. lentus, 23 S. epidermidis, and 17 S. xylosus) were isolated from four dairy goat farms in central and south Taiwan. Staphylococcal incidence were higher in milk, vagina, anus, and nasal cavity than in other isolation sources. It seemed that staphylococci may survive in dairy goat farms extensively. PCR detection of enterotoxin genes, sea~see and seg~sej, found sec in 25 S. aureus and see in 1 S. aureus, of 98 clinical staphylococcal strains and 86 S. aureus previouly isolated in laboratory, and no enterotoxin genes in any CNS. PCR-RFLP analysis of sec PCR products only presented single type. Among 10 S. aureus isolates collected from goats with clinical mastitis, 8 S. aureus consisted of sec. These results suggest that SEC may be a main type to S. aureus of goat, and sec may be an important virulence factor to S. aureus goat mastitis. Antibiotic sensitivity test showed Staphylococcus spp. Had serious resistance to penicillin G, ampicillin, tetracycline, and oxytetracycline, and most isolates were multidrug-resistant strains. In the result of PCR detection to methicillin resistant genes of S. aureus and S. lentus, it was found that 9 MRSA (methicillin-resistant S. aureus) strains with SCCmec III, 2 MRSA strains with SCCmec II. PFGE (pulsed-field gel electrophoresis) analysis of MRSA collected from goat and human found similar PFGE patten, suggesting that S. aureus may transmit between goat and human.