Development of FPV's env Gene as a Subunit Vaccine

• Main Author: 王智弘
• Other Authors: 張文興
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/79169683349076812953

碩士 === 國立嘉義大學 === 生物農業科技學系碩士班(Institute of Agricultural === 98 === Abstract Chicken is one of the most important sources of animal proteins in the world. Infection of fowlpox virus has been demonstrated to cause the skin inflammation and lesion in chicken. The infected chicken will lose its weight, reduce egg production, and even die. The situation of virus infection can cause great loss to the chicken raisers. To prevent the fowlpox infection, live attenuated viral vaccines are routinely applied in the chicken farm. However, the application of live vaccinia virus poses a threat of virulent regression. In this study, the envelop gene of fowlpox virus is cloned, sequenced, and expressed in E.coli. The expressed viral protein is purified by His-tag column and confirmed by SDS-PAGE and Western blotting assays. The viral protein is further characterized by immunization and viral challenge assays to determine its effectiveness as a subunit vaccine. Finally, the newly developed vaccine is hoped to provide a safer and better approach to fight against the fowlpox infection in the chicken farm.