The production of Cordyceps fermented products and its improvement on the production performance and egg quality in laying hens

• Main Authors: Yong-Cheng Chen, 陳勇成
• Other Authors: Kuo-Lung Chen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/13130722263976636056

碩士 === 國立嘉義大學 === 動物科學系研究所 === 98 === The purpose of this study is to investigate the production of winter worm summer grass fermented products by solid-state fermentation, and the effect of feed supplemented with winter worm summer grass fermented products on the production performance and egg quality in laying hens. Part I: Sixty, 42-week-old Single Comb White Leghorn laying hens randomly allocated into four dietary treatments: supplementation with 0, 0.1, 0.3 and 0.5% of Acremonium implicatum fermented products (AIFP) with three replicates for a 11-week feeding trial. The results showed that egg production and feed efficiency of 0.3% groups were better than the control (P<0.05) in whole experimental period. The height of albumin and Haugh unit (HU) of 0.1 and 0.3% groups were higher than the control after 3 d of storage (P<0.05). HU of 0.1% was higher than the control after 9 d of storage (P<0.05). The albumin index and HU of 0.3% were also higher than the control after 15 d of storage (P<0.05). Part II: In the trial 1, 50g broiler feed or wheat was used as fermentation substrate mixed 15 ml Cordyceps militaris culture and then adjusted water to 1: 0.9 of total water-added (water and culture). The better mycelium growth results showed in wheat. In trial 2, wheat was used as fermented substrate. to find out the best β-glucan content in different moisture content, period of fermentation, pH value, and fermentation temperature. The result shows moisture ratio 1:09, 21 days fermentation, pH 6.2, and 20℃ could get the best β-glucan content. In trial 3, wheat, oat and barley were used as fermented substrate. β-glucan of barley-based fermentation products β-glucan content of 21d-cultured fermented production was highest. Part III: One hundred 24-week-old Single Comb White Leghorn laying hens (Hy-Line W36) were selected and randomly allocated into five supplementary treatments with five replicates, namely, 0.3% AIFP, Cordyceps militaris wheat (CMW), oat (CMO) and barley (CMB) fermented products for a 25 weeks feeding experiment. In 1-11 weeks of experimental period, the egg production of AIFP group was significantly higher than the control (P<0.05). The egg weight of CMB group was significantly higher than the control and AIFP (P<0.05). The egg mass of all treatment groups were higher than the control (P<0.05). In 12-25 weeks of experimental period, the egg mass of CMB group was highest (P<0.05). In whole experimental period, the egg production of AIFP group was significantly higher than the control (P<0.05). The egg mass of CMW, CMB and AIFP groups were higher than the control (P<0.05). The albumen height, albumen index, HU, and albumen pH had treatment effect (P < 0.05) and storage effect (P < 0.0001) at 6th week storage trial. CMB group had higher albumen height, albumen index and HU than those of control and the albumen pH was lowest after 3 and 9d storage (P<0.05). AIFP had higher albumen height, albumen index and HU than those of control and albumen pH was lowest after 9d storage (P<0.05). The albumen height, albumen index, HU, and albumen pH had treatment effect (P < 0.05) and storage effect (P < 0.0001) at 12th storage trial. CMB and CMO groups had higher albumen height, albumen index and HU than those of control and albumen pH was lowest after 3 and 9d storage (P<0.05). However, only CMB had lower albumen pH than those of control after 9d storage (P<0.05). AIFP had higher albumen height after 9d storage (P<0.05). In conclusion, it could get the best CMW β-glucan content under the condition of substrate and moisture ratio was 1:0.9 (w/v), 21days fermentation, pH 6.2, and 20℃The CMW, CMO, and CMB β-glucan content were 6.9, 7.7, 13 %, respectively. Dietary supplementation with the 0.3% winter worm summer grass fermentation products can improve production performance in laying hens. Supplementation with CMB and AIFP fermented products can improve the egg quality and extend the egg shelf life during storage, however, it is still need further research the improve production performance and egg quality mechanism of winter worm summer grass.