Study different forms of type I collagen on regulating differentiation and proliferation of human placenta derived multipotent cells

• Main Authors: Chien Ke, 柯傑
• Other Authors: Cheng-Nan Chen
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/14603605316309055639

碩士 === 國立嘉義大學 === 生化科技學系研究所 === 98 === Type I collagen constitutes a major portion of the extracellular matrix (ECM) in arterial wall and it is the major substrate for cell growth and differentiation. The goal of this study was to evaluate the differentiation and proliferation of placenta-derived multipotent cells (PDMCs) on polymerized type I collagen fibrils and monomer collagen. PDMCs grown on both polymerized collagen and monomer collagen with transforming growth factor (TGF)- treatment increases the expression of smooth muscle cell (SMC)-specific markers, including a-smooth muscle actin (a-SMA) and calponin. Polymerized collagen increased the expressions of p21CIP1 and p27KIP1; decreased cyclin A, cyclin D1, cyclin-dependent protein kinase 2 (Cdk2); and led to G0/G1 arrest in PDMCs. Thus, polymerized collagen exhibits the great potential in inducing PDMCs differentiation into SMCs, and exerts anti-proliferative effect on PDMC-differentiated SMCs.