Studying ethylene biosynthesis in Arabidopsis thaliana by activation tagging approach

• Main Authors: Chia-ping Liu, 劉佳苹
• Other Authors: Shaw-Jye Wu
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/01754891952492706423

碩士 === 國立中央大學 === 生命科學研究所 === 98 === Ethylene is a gaseous phytohormone and has a versatile role in plant physiology. Ethylene biosynthesis is committed by the conversion of S-adenosylmethionine (SAM) to 1-aminocyclopropane-1-carboxylic acid (ACC) by ACC synthase (ACS). Previous studies showed that ETO1 (ETHYLENE OVERPRODUCER1) negatively regulated ethylene biosynthesis and resulted in increased ethylene production in Arabidopsis thaliana. ETO1 represents a gene family in Arabidopsis thaliana, including EOL1 and EOL2 (ETO1-LIKE). There are two distinct protein domain in ETO1. BTB (Broad-Complex, Tramtrack, and Bric a brac) and TPR (Tetratricopeptide Repeat) domains, which both are involved in related with protein-protein interactions. My thesis research is to use activation T-DNA tagging approach to identify suppressors of eto1-5 in Arabidopsis thaliana. By screening the activation tagging mutants carrying an eto1-5 allele, we have found 16 seat (suppressor of eto1-5 by activation tagging) mutant lines. All of the 16 seat mutants are dominant and belong to at least 3 complementation groups. I have identified the T-DNA insertion sites in the seat mutants to analyse how the flanking genes regulate seedling phenotype of eto1-5. In the future work, Arabidopsis transgenic lines will be generated to confirm the function of selected genes as suppressors of eto1-5.