Fabrication of molecularly imprinted photonic crystals for detection of bisphenol A

• Main Authors: Chen, Tzu-Yin, 陳姿吟
• Other Authors: Chang, Sue-Min
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/04891291888147965805

碩士 === 國立交通大學 === 環境工程系所 === 98 === Bisphenol A (BPA) is an endocrine disruptor which could cause hormone-related cancers. In this study, a novel imprinted photonic crystal (IPC) was developed for the detection of bisphenol A (BPA). The shifts in the wavelength of the diffraction peaks, resulting from the changed refractive index (n) of the IPC after rebinding of BPA, were adapted for the quantification of the target compound. The molecularly imprinted polymer (MIP) was prepared using a sol-gel method in which zirconium propoxide (ZPO) was used as the cross-linker and phenyltrimethoxysilane (PTMOS) was used as the functional monomer which bound BPA via π-π stacking interaction. The MIP with the PTMOS/BPA molar ratio of 1 had the high adsorption capacity of 4.62 mg/g and imprinted factor of 20.8. Inverse opal photonic crystal was prepared using polystyrene (PS) microspheres (163 nm) as the template. Hexagonal PS colloid crystals was formed through a heat-assisted self-assembly method at 50oC. After infiltration of the colloidal crystals with 2.5 ?尳 imprinted sol solution, the PS microspheres were subsequently removed using solvent extraction. The obtained inverse opal ZrO2 exhibited a photonic bandgap of 4.8 eV (l = 257 nm). The IPC prepared with the ZPO/PTMOS/BPA/EtOH molar ratio of 30/1/1/80 exhibited the optimal microstructures for the largest wavelength shift of 3.2 nm at 50 mg/L BPA. The detection can be completed within 4 min. In addition, a small variation of 4.6% was obtained in 5 detection cycles. The linear detection range in pure water and the raw water from Pao-Shan reservoir was 1-60 and 1-100 mg/L, respectively. The IPC performed insignificant response for BPA analogues including phenol, 1-naphthol and 4-tert-butylphenol (BP), indicating its high selectivity. These results clearly demonstrated that the IPC is an advanced sensing device which can be applied for in-situ and on-site analysis in the future.