Infrared Spectroscopic Studies on the Self-Association of 2-(trichloroacetyl)pyrrole and its Hetero-Association with Toluene in Various Solvents

• Main Authors: Wu, Hong-Bin, 伍竑賓
• Other Authors: Chen, Jenn-Shing
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/59806609084374251623

碩士 === 國立交通大學 === 應用化學研究所 === 98 === The advent of biotechnology has drawn much attention to the public. In biotechnology, the function, structure, and reaction of proteins play a crucial role. As to the structure, it is the N-H•••O=C hydrogen bonding that is a major factor in stability. Due to the similar hydrogen bonding involved between 2-(trichloroacetyl)pyrrole molecular dimerization, it is chosen as a model for the investigation into the protein stability. The FTIR has been employed to this purpose. The fact that the spectra of associated dimer and free monomer N-H stretching modes appear concurrently allows, upon deconvolution, the data of the integrated absorbance of the monomer is to be fitted into the linear equation (2-9), page 14, to determine the monomer molar absorptivity, dimerization constant; and that of dimer is to be fitted into equation (2-12), page 15, to determine the dimer molar absorptivity and dimerization constant at various temperatures. Since the very same dimerization constant can be determined by different data sources, the disparity of the results provides a criterion for judging the consistency of the determination. The enthalpy and entropy of dimerization can be obtained via a van’t Hoff plot. Instead of in a linear form, the dimer is predominantly in a cyclic form, this is reasoned from a plot based on a linear equation (3-28), page 121. Also the hetero-association of the 2-(trichloroacetyl)pyrrole with toluene has been studied to obtain the complex molar absorptivity and complexation constant using equation (2-20), page 19. The enthalpy and entropy of complexation have also been determined.