Hydrogel films for releasing and sensing signaling molecules

• Main Authors: Huang, Yi-Sa, 黃以撒
• Other Authors: Meng, Hsin-Fei
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/12773806495954002940

碩士 === 國立交通大學 === 物理研究所 === 98 === Signaling molecule is an important substance that transmits information in human body. The concentration of signaling molecule changes very fast and hence it is very interesting to produce or detect such a fast changing molecule. In this dissertation, two kinds of hydrogel films are fabricated for producing nitric oxide (NO) and detecting zinc ions. In the first part of this dissertation, an optically controlled solid-state nitric oxide donor with hydrogel matrix is realized for generating NO pulse. The solid-state NO donor is a film composed of host polymer poly(2-hydroxyethylmethacrylate) (pHEMA) and donor molecule sodium nitroprusside (SNP). The pHEMA traps the SNP tightly so the leakage of SNP or the residue of SNP is diminished. The solid-state NO donor is stable in the solution without laser excitation. After being excited by laser, the solid-state NO donor releases NO into the environment. The NO release is monitored by the NO probe 1,2-diaminoanthraquinone (DAQ) whose decrease in absorption can be observed after reacting with NO. The amount of NO that released from the solid-state NO donor after six hours laser excitation is estimated to be 1.83x10-10 mole. The second part of this dissertation demonstrates a sensing hydrogel film for zinc ion. The solid-state zinc sensing hydrogel film is a film composed of host polymer poly(2-hydroxyethylmethacrylate) (pHEMA) and probe molecule m-Benziporphodimethene (BPDM-H). The pHEMA tightly traps the BPDM-H without affecting the photoluminescence (PL) spectrum of BPDM-H. Almost no PL intensity can be observed when there is no zinc ion in the liquid environment. As zinc ion is added into the environment, the PL intensity of the zinc sensing hydrogel film increases with time. The lowest zinc concentration detection limit is 10-4 molar concentration.