| Summary: | 博士 === 國立交通大學 === 材料科學與工程學系 === 98 === What is the specialty of bio-toxicity caused by nanoparticles? How is the interaction between nanoparticles and bio-molecules? And how do we use this specialty in further applications? Nanotechnology has been widely developed in recent years and has been used for fabrication in high-quantity of nanoparticles, which remains in the environment and also endanger to the livings. Many researches have revealed that when the size of the matter decreases to nanoscale, matter would invade into the livings and would cause severe inflammation and bio-toxicity. We have investigated the interaction between nanoparticles and bio-molecules, to help and understand the toxicity of nanoparticles and preventing the damage. Gold nanoparticles have the best biocompatibility and we can precisely control the size of gold nanoparticles. Because of the unique size effect leading to bio-toxicity and interesting interaction between nanoparticles and biomolecules, a number of serious concerns have been raised about what effects these will have on our society if realized, and what action if any is appropriate to mitigate these risks.The major theme of this thesis can be separated into 9 sections:
1. Assessment of the in vivo toxicity of gold nanoparticles: we found that The toxicity of GNPs may be a fundamental determinant of the environmental toxicity of nanoparticles.
2. Gold nanoparticles pass through the blood-brain barrier and impair learning and memory in mice: we found that the ability of GNPs to damage cognition in mice is size-dependent and is associated with their ability to invade the hippocampus.
3. Measuring the flexibility of immunoglobulin by gold nanoparticles: we found a novel platform to measure the functional flexibility of immunoglobulin.
4. Contribution of hinge flexibility to the antibody/ nanoparticle recognition: we found that two levels of affinity selection were disclosed. It is obvious that there exist only certain structural ranges that would allow the best binding between 2 FAB fragments and the surface of 5-nm GNP.
5. Detection of gold nanoparticles using immunoglobulin-coated piezoelectric sensor: we provide an immunoglobulin-based nanoparticle sensor thus provides a direct and economical solution.
6. Antibody-guided nanofabrication: inserting silicon nanowires into nanopores: we made use of antibodies that interacted with gold nanoparticle-coated nanowires to guide the insertion into nanopores. The successful application of antibody-antigen interactions provides a novel approach to manipulate objects at the nanoscale.
7. Label-free and ultra-sensitive detection of the cymbidium mosaic virus with an antibody-functionalized nanowire field effect transistor: we found that the detection of CymMV provides an ultra-sensitive and time-efficient platform for the protection of Orchidaceae from this virus.
8. Assessment for the size-dependent poperties of gold nanoparticles as a vaccine carrier to elicit a focused and enhanced antibody response against synthetic Foot-and-mouth disease virus peptide: we found that GNPs ranging from 8 nm to 17 nm may serve as an ideal carrier to elicit focused antibody response against a synthetic pFMDV peptide.
There has been a great progress in the field of nanotechnology which impacts every field of science as well as in our daily life. An understanding of nanotoxicity and bio-nano interaction could lead to the harnessing of nanotechnology properties. The nanotechnology will bring much more convenience to our life.
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