Establishing a Computational Pipeline of Genome Projects: Sequence Assembly, Gene Annotation and Metabolic Pathway Reconstruction

• Main Authors: Huang, Chih-Chang, 黃至昶
• Other Authors: Huang, Hsien-Da
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/30090000954245955675

碩士 === 國立交通大學 === 生物資訊及系統生物研究所 === 98 === Human Genome Project had been completed in 2003. It provides gigantic resources for biological research. In recent years, next generation sequencing technique dramatically reduces the sequencing cost and time. Thus, completely sequencing new organisms will be popular and universal, and the genomes of these organisms also include huge research resources. The demands of comprehensive genomic annotation will be more urgent and necessary. Thus, it is necessary a computational pipeline. In order to assembly complete genome sequences, this pipeline uses several assembly tools which designed for assembling traditional sequencing and next generate sequencing raw data. It also integrates ab initio and evidence-based gene prediction approaches to predict genes. In addition, this pipeline can reconstruct metabolic pathways from the gene annotation results. This computational pipeline can assemble sequencing data from various platforms and provide the service of genomic annotation including: gene annotation and metabolic pathway reconstruction. This computational pipeline can be a crucial part of pipeline in the high throughput genomic annotation.