The regulatory effects of crude extracts derived from Corbicula fluminea on inflammation

• Main Authors: Lin, Ching-Min, 林靜敏
• Other Authors: Liao, Kuang-Wen
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/78783116672385602606

碩士 === 國立交通大學 === 生物科技學系 === 98 === Corbicula fluminea（Freshwater clam）is the major fresh water cultivated bivalvia in Taiwan. It is used to be as a remedy for liver diseases such as hepatitis. Recent reports indicate that the hot water extracts of C. fluminea could reduce liver injury induced by ethyl alcohol. However, whether C. fluminea has any influence on inflammation is still unclear. For evaluation of C. fluminea’s anti-inflammatory activity, masses of the claim were respectively extracted by chloroform or hot water to obtain the clam chloroform extracts (CCE) and hot water extracts (CWE). Briefly, CCE and CWE were examined their influences on proinflammatory responses in this study. Firstly, the results revealed that neither CCE nor CWE could induce changes in proliferation of human mononuclear cells and the releases of proinflammatory cytokines. However, when the inflammatory responses were triggered by lipopolysaccharide (LPS), CCEs significantly displayed their anti-proinflammatory activities to lower the releases of LPS-induced proinflammatory cytokines through suppressing mRNA expression. Unlike CCE, CWEs showed their minor and controversial actions on the releases of LPS-induced proinflammatory cytokines. Sequentially, CCEs were further verified their anti-inflammatory activities in vivo and the results were consistent with the actions of CCEs in vitro. Finally, the CCEs were determined their components by GC-MS and the results exposed that there were at least 6 compounds in CCEs with various anti-inflammatory activities according to the published literatures. Thus, this study revealed C. fluminea has the anti-inflammatory capability and indicated that the compounds in CCE play the key roles of anti-inflammatory activity.