| Summary: | 碩士 === 國立成功大學 === 物理治療研究所 === 98 === Background and Purpose: Diabetic patients have impaired sensation and easily result in serious infections which cause endotoxemia. Type 1 diabetic patients require some early interventions to prevent complications. Physical exercise is one kind of interventions that is capable to change the level of inflammatory cytokine (interlukin-6; IL-6) and anti-inflammatory cytokine (interlukin-10; IL-10). Exercise training also influences the apoptotic process and the expression of heat shock protein (HSP). Therefore, the objective of this study was to revel whether streptozotocin(STZ)-induced diabetic rats tend to change endotoxemia-induced inflammation, apoptotic status after exercise training and detraining. Method: Wistar rats (8 wk old) were randomly assigned to normal and diabetic group. Each group was arranged into three subgroups: sedentary, exercised and detraining groups. The rats in the exercised-group ran on a treadmill 5 day/week, 30-60min/day with intensity 20-25 m/min for 4 weeks. The detraining group was detrained for 10 days after 4-week exercise training. Twenty-four hours later, Lipopolysacharide (LPS) or saline were then injected intravenously. At the 3rd hour after LPS injection, we determined IL-6 and IL-10 in serum and lavage; caspase-1, caspase-3 and HSP72 levels in lung and heart. Result: the body weight in STZ-induced diabetic rats with exercise group were significantly higher than STZ-induced diabetic group (p = 0.041). The blood glucose in exercised-diabetic group were significantly lower than diabetic group (p = 0.026). IL-6 and IL-10 levels in serum of LPS group were significantly higher than saline group (p＜0.001). After administration of LPS, serum IL-6 level in the normal-exercised group showed a significant decrease compared to the normal-sedentary group (p=0.034). The serum and lavage levels of IL-6 in detraining group were significantly higher than exercise and sedantary groups. However, exercise could slightly decrease serum IL-6 level in the diabetic groups. The expression of HSP72 was significantly increasing after exercise. Caspse-1 and caspase-3 levels didn’t change significantly after exercise training and detraining. Conclusion: The results indicated that exercise training seemed to suppress the inflammatory status. However, the inflammatory status increased in detraining groups. Clinical Relevance: Exercise training may attenuate the inflammatory status during endotoxemia. Our results suggested that type 1 diabetic patient should keep an active life style.
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