Study on Self-Assembly Behavior of Modified Gold Nanoparticles with Oligonucleotides by Using Dissipative Particle Dynamics (DPD)

• Main Authors: Wei-ChengHuang, 黃偉誠
• Other Authors: Tei-Chen Chen
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/72013211234945101032

碩士 === 國立成功大學 === 機械工程學系碩博士班 === 98 === In this paper, self-assembly behavior of modified gold nanoparticles with oligonucleotides was studied by using dissipative particle dynamics(DPD) . During the simulation, the influence of the appropriate size of simulation box, the aggregation of different morphology of the DNA-AuNPs and the different length of DNA chain on the resulting cluster size and the change of morphology was investigated individually. The results showed that the mean aggregation number change of the the DNA-AuNPs of different morphologies with the DNA spacer, completely grafted on gold nanoparticles is dependent upon whether the chain is perfectly extended or not.When the volume fraction of beads of hybridization is close to fifty-fifty, the mean aggregation number will increased dramatically. As the DNA chain was enlarged, the ratio of the DNA chain and the diameter of AuNPs increased, while the mean aggregation number does not increase linearly.Moreover, as the oligonucleotide numbers is increased to 15-mer, attributed to the soft flexibility of DNA spacer, the DNA spacer exhibited tangling phenomenon with inefficient hybridization. It was found that as the DNA chain length increased, the overall cluster structure will transforme from ellipsoid-like to spheroid-like by using radius of gyration and asphericity.