| Summary: | 碩士 === 國立成功大學 === 生物資訊研究所 === 98 === Znf179 is a member of the RING finger protein family. During embryogenesis, Znf179 is restrictedly expressed in brain region, suggesting a potential role in neuronal development. Previous studies in our laboratory have demonstrated that the expression of Znf179 is remarkably increased in the retinoic acid-induced neuronal differentiated P19 cells and Znf179 knock-down significantly attenuates neuronal differentiation. To investigate the molecular mechanisms of Znf179 function, we performed yeast two-hybrid screen to identify the Znf179 interacting proteins. In the experiments, promyelocytic leukemia zinc-finger (PLZF) was identified as a novel Znf179 interacting protein. The results of immunoprecipitation assay also confirmed the interaction between PLZF and Znf179 in vivio. Furthermore, we have demonstrated that PLZF interacts with Znf179 via its two zinc-finger domains, which is located between amino acids 398 and 455. Using immunofluorescence analysis, we observed that the cellular localization of Znf179 was changed by co-expressing PLZF. Taken together, these results indicate that the two proteins dose indeed interact with each other in vivo. PLZF has been found to regulate transcription through recruitment of nuclear receptor corepressors (N-CoR or SMRT) / histone deacetylase (HDAC) complexes via its POZ domain. To investigate whethere Znf179 affects transcriptional regulation ability of PLZF, COS-1 cells were co-transfected with HA-PLZF and EGFP-Znf179 or control plasmid. Unexpectedly, we found that exogenously expressing Znf179 caused a 5-fold increase in HA-PLZF protein abundance but not at Flag-PLZF RNA level. Further studies will be performed to examine the molecular mechanisms of Znf179 functions on PLZF protein regulation.
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