Neuronprotection of MA-1-PS in vitro：therapeutic window and anti-excitotoxicity, antioxidant as well as free radical-scavenging action

• Main Authors: Chiao-WenHuang, 黃巧雯
• Other Authors: Tian-Shung Wu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/09168701651144871997

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 98 === Abstract Cerebral stroke is a leading cause of death worldwide. Because of the lack of efficient therapeutic alternatives, induced to transient focal cerebral ischemia that occurs during cerebrovascular surgery may result in irreversible cell damage and neurobehavioral dysfunction due to the release of excitotoxic neurotransmitters, influx of calcium ions into neurons, free radical generation, lipid peroxidation, and protein degradation. They eventually lead to necrotic and/or apoptotic cell death. MA-1-PS is a derivative of MA-1. MA-1, a natural compound isolated from Cinnamomum philippinense. The agent is highly lipophilic. In the past researches have been demonstrated MA-1 was a strong natural antioxidant and free radical scavenger, had the potential to cross the blood-brain barrier to the brain and had neuroprotective properties. But when a serious patient is in a stupor, can not to oral drug by himself, so to modify MA-1, the product is MA-1-PS, is water soluble, moreover improve the solubility, can be easy to supply intravenous injection for therapy. To confirm MA-1-PS whether has the same efficiency of neuroprotection. In experiment model, we use primary neural culture and organotypic hippocampal slice cultures, combined with oxygen-glucose deprivation ( OGD ), to mimic ischemia closely the situation in vivo result in brain disease. Further, to demonstrated that MA-1-PS have neuroprotection of ability via inhibiting lipidperoxidation and scavenging free radical.