| Summary: | 碩士 === 國立成功大學 === 化學工程學系碩博士班 === 98 === Human urinary albumin plays an important role in the clinical diagnosis of kidney disease. The examination of urine albumin can help medical staff determine kidney function and seriousness of disease. In this study, an electrochemical urinary albumin sensor was proceduced. It mainly used cyclic voltammetry and ac impedance analysis to detect peak potential, peak current and impedance in different concentrations and adsorption times of albumin, and pHs of electrolyte in a Fe(CN)63- / Fe(CN)64- system. SAM modified electrodes were also applied to increase sensitivity of the detection.
The result shows the feasibility by using EDC and NHS to stabilize the modification layer of electrode surface. According to the results from the SAM modified and bare electrodes, the reactions on electrodes both before and after modification were all irreversible eletochemical reaction. Peak potential and peak current ratio changed with adsorption time and became stable after 40 minutes. The sensitivity of peak potential difference was 6.260 mV/ppm with bare electrode, and was improved to 7.398 mV/ ppm with SAM electrode. When albumin concentration increased, the irreversibility increased and activity of electrode decreased. The sensitivity of peak current ratio was 1.324 μA/ppm (oxidation) and 1.506 μA/ppm (reduction) with bare electrodes, and was improved to 1.703 μA/ppm (oxidation) and 1.831 μA/ppm (reduction) with SAM electrodes. Rs and Rct increased with the albumin concentration and adsorption time.
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