The Effect of Oblique Ultrasound on Fibroblasts and Bovine carotid artery endothelial Cell migration

• Main Authors: Chia-Yi Lin, 林家億
• Other Authors: Chun-Mei Lu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/09814221452564501836

碩士 === 國立勤益科技大學 === 化工與材料工程系 === 98 === Ultrasound is a directional transfer of energy and force, so the role of micro-streaming can be divided into horizontal and vertical direction. Therefore, this experimental study ultrasound force to simulate the environment within the capillary to construct a horizontal direction of the ultrasonic micro-flow field, the cells stimulate the different levels of force and to observe differences in cell migration. Cell migration is fundamental cellular process for normal development and homeostasis of tissue and organ. In study, horizontal flow fields were set up by 0.05 W/cm2 and different angles (40O, 70O and 90O) of ultrasound emitting. The migrations of fibroblast and Bovine carotid artery endothelial cells were observed within 8 hours. Uses the aseptic needle to blow except the cell, causes it to present a non-cell region, in standing upside down on the type microscope to erect the supersonic wave to stimulation, the supersonic wave stimulates 5 minutes by each hour, carries on eight hours and photographs the observation. Finally, immunofluorescence staining of cytoskeleton、 vinculin, and nucleus.