Cytopiloyne regulated adipogenesis by maintaining GATA-3 expression and affecting the PKCα pathway

• Main Authors: Nai-Chen Pan, 潘乃蓁
• Other Authors: Lee-Tian Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/30805688811011883482

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 98 === Certain nature compounds have ability to inhibit adipogenesis and induce apoptosis in adipocytes. Adipocyte differentiation involves a temporally regulation set of gene-expression events, such as peroxisome proliferator activated receptor-γ (PPARγ) and GATA binding protein-3 (GATA-3). In our investigation, we found cytopiloyne (CP), a phytocompound from Bidens pilosa, can reduce the differentiation of adipocytes from various animal sources. We also proved CP inhibits adipocyte differentiation through the GATA-3 pathway in 3T3-L1 cells. Our previous results showed CP can regulate the expression of PKCα translocation in RAW264.7 macrophages and RIN-m5F β-cells. Recent papers indicated that the expression of PKCα and calreticulin (CRT), the major Ca2+-binding protein, can regulate the adipogenesis in 3T3-L1 cells. Therefore, we will focus the adipogenic effect of CP on interaction among PKCα, CRT and Ca2+.