| Summary: | 博士 === 國立中興大學 === 食品暨應用生物科技學系所 === 98 === This dissertation research concerns the antioxidant capacities of mulberry (Morus spp.) and the antimetastatic potentials of 1-deoxynojirimycin (1-DNJ), which is rich in mulberry. First, five genotypes (Miaoli No. 1, 73C020, 46C019, 74H3023, and 68H22024) and three maturity stages (unripe, medium ripe and fully ripe) of the mulberry (Morus sp.) fruit were analyzed by different antioxidant assay and principal component analysis. The results provided the basic data for choosing the genotype of mulberries with higher antioxidant activities. Second, we hypothesized that 1-DNJ could prevent metastasis of melanoma cells via the directory inhibitory effects and/or the immune-enhancing effects on cancer cells. To examine above hypothesis, we used a highly invasive melanoma cell line, B16F10 cells, and we found that 1-DNJ significantly inhibited the invasion, migration and adhesion, and that the effect was likely associated with attenuated activities and expression of matrix metalloproteinases (MMP)-2/9, enhancement of the tissue inhibitors of metalloproteinase (TIMP)-2 mRNA expression, and the alterations of the cell surface-binding motif. In addition, we further showed that 1-DNJ-conditioned media (1-DNJ-CM), which were obtained from the incubation of mouse splenocytes with 1-DNJ, significantly suppressed the invasion, migration and adhesion. The effect of 1-DNJ coincided with enhancement of splenocytes proliferation and the mRNA expression of cytokines (IL-2, 24, TNF-α, IL-8 and IL-10). The results stated above suggest that the antimetastatic potential of 1-DNJ may result from both the direct inhibitory and the immune-enhancing effects on cancer cells. It should be noted that the 1-DNJ concentrations required for the direct inhibition are much higher than those required for the immunomodulatory effect. Taken together, we conclude that immunomodulatory effect of 1-DNJ may play a more important role than the direct inhibitory effect on the antimetastatic activity against B16F10.
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