| Summary: | 碩士 === 國立中興大學 === 昆蟲學系所 === 98 === Application of DNA sequences in molecular systematics has become more prevalent in the previous decade, not only in taxonomic work, but also in many relative researches focusing on phylogeny, population genetics, and evolutionary events. In 2003, the so-called “DNA barcode” was proposed and allocated to identify organisms based on sequences of the mitochondrial COI gene (cytochrome oxidase subunit I). The study herein, DNA barcodes of Taiwan lepidopterans were established, then used to discuss the sequence divergences in each taxonomic category. The samples applying in this study were majorly derived from National Museum of Nature Science, and including some saturniids were collected from field. The result, in general, shows that the intraspecific sequence divergences are lower than 2%. However, limited cases of sequence variation lower than 2% are also found between species within a given genus and between genera within a given family respectively. Furthermore, we compiled relative sequences from NCBI database and found the sequences divergence are 4~14% and 6~17% in specific and generic categories, respectively; and the result shows the overlapping ranges of the above two categories from 7~10%. Sequences divergence in several cases less than 2% between generic species is appearing in Noctuidae, Bombycidae and Saturniidae; moreover, as comparing to other saturniid subspecies, sequences show a great divergence in subspecies collecting from Taiwan. In conclusion, barcoding analysis in this study does show the species identification possibility in additional to discover the cryptic species or refine the species resolution.
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