| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 98 === Objective: Uterine cervical cancer has high mortality and should be early detected, diagnosed and treated for the purpose of better prognosis. At present, human papillomavirus (HPV) is the most important and necessary cause of cervical cancer. Therefore, HPV detection in cervical specimens and HPV preventive vaccination are important in cancer prevent. This study is aimed at the HPV genotype survey in Taiwan to predict the efficiency of HPV vaccination. COX-2, as an inflammatory indicator, is also stained by immunohistochemical method to evaluate the correlation with HPV infection.
Materials and methods: One hundred and forty-one paraffin blocks were enrolled for the HPV genotyping by EASYCHIP HPV Blot and COX-2 immunohistochemistry stain. Results: In the 141 cases, 98 cases (98/141，69.50 %) were positive for HPV DNA , as inflammation: 40.00 % , mild dysplasia: 44.00 % , moderate dysplasia: 44.44 % , severe dysplasia: 78.57 % , CIS: 84.62 %, cancer: 77.42%. In 98 HPV DNA positive cases, 78 cases (79.59%) had single type infection and 20 cases (20.41%) had multiple type infection. The first five single genotypes were HPV 16 (23.47%), 58 (13.27%), 52 (10.20%), 18 (5.10%) and 70 (4.08%), respectively and were 56.12 % in total. In multiple type infection, 95 % had lesions of CIS or cancer. In the 98 HPV positive cases, COX-2 IHC stains show 74 positive stain (75.51 %), 24 negative stain (24.49%). In the 43 HPV-negative cases, 30 cases (69.77%) show COX-2 positive stain and 13 cases (30.23%) show negative stain.
Conclusion: The commercial HPV vaccine covers genotypes 6, 11, 16, 18 but not covering the first five genotype, such as 52, 58, 70, in Taiwan (covering rate was 33.67 %). There is no significant correlation between the HPV and COX-2, suggestive of HPV infection was related to cervical inflammation and the succeeding carcinogenesis but not strongly related to the tumor progression and tumor growth factors (COX-2).
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