Antioxidant properties of Phellinus igniarius mycelia by solid-state fermentation using wheat oats and buckwheat as a substrate

• Main Author: 鄭亦紜
• Other Authors: 龍明有
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/72642164738314892357

碩士 === 明新科技大學 === 化學工程與材料科技系 === 99 === Phellinus igniarius, also known as Sang-Huang mushroom, is often grown in the living Mulberry. P. Igniarius likes a black tumor with black color and hard fruiting body as well as the deformation of the trunk material, which is characterized by the appearance on the dark brown and bright yellow inside the fruiting body. P. Igniarius possesses mainly anti-tumor effect and other medicinal value, including immunomodulatory, anti-angiogenic, anti-mutagenic, anti-cancer, liver, liver fibrosis and the hypoglycemic effect. Therefore, the purpose of this study is to use a substrate including wheat, oats and buckwheat as a substrate by using solid-state fermentation (SSF) to produce P. Igniarius mycelia, and antioxidant properties of the extracts from P. Igniarius mycelia were examined. The results told that ethanol extract from mycelia by buckwheat SSF showed an antioxidant activity of 63%. For wheat SSF, ethanol extract from mycelia revealed a reducing power of 0.49. With regard to wheat SSF, polysaccharide extract from mycelia revealed a reducing power of 0.48. The hot water extract from mycelia by oats SSF exhibited a scavenging DPPH radical ability of 70%. The ethanol extract from mycelia by oats SSF exhibited a chelating ability of 80%. The polysaccharide extract from mycelia by oats SSF also exhibited a chelating ability of 80%. With regard to buckwheat SSF, polysaccharide extract from mycelia revealed a scavenging superoxide radical ability of 60%. The lowest EC50 values are described as follows: for antioxidant activity, EC50 values of ethanol extract from mycelia by buckwheat SSF are 6.44 mg mL-1. For reducing power, EC50 values of polysaccharide extract from mycelia by oats SSF are 0.14 mg mL-1. For scavenging DPPH effects, EC50 values of hot water extract from mycelia by oats SSF are 6.07 mg mL-1. For chelating ability, EC50 values of polysaccharide extract from mycelia by buckwheat SSF are 2.92 mg mL-1. For scavenging superoxide radical ability, EC50 values of ethanol extract from mycelia by wheat SSF are 6.91 mg mL-1. The polysaccharide content of mycelia from wheat and buckwheat SSF is 200 mg mL-1. The hot water extraction yield of mycelia by oats SSF is 70.5%. Based on results obtained, P. Igniarius mycelia produced by wheat, oats and buckwheat SSF possess better antioxidant properties, and can act as a source potential for application in health food.