Determination of piperacillin in human plasma and peritoneal fluid by capillary electrophoresis coupling with large volume sample stacking

• Main Authors: Mu-Jung Sun, 孫慕容
• Other Authors: CHEN,SU-HUI
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/29134458257519863382

碩士 === 高雄醫學大學 === 藥學研究所 === 98 === A sensititive high performance capillary zone electrophoresis (CZE) combining on-column large volume sample stacking (LVSS) injection has been developed for determination of piperacillin in human plasma and peritoneal fluid. A sample pretreatment by means of liquid-liquid extraction (dichloromethane) with subsequent quantitation by LVSS-CZE was used. The separation of piperacillin was performed using a background electrolyte containing 60 mM phosphate buffer (pH 6) with 0.005% polyethyleneoxide (PEO) as a dynamic coating capillary wall to inhibit electroosmotic flow (EOF). The sample was hydrodynamic injection (0.5 psi, 70s) to introduce sample and the applied voltage was -15 kV and on-column detection at 200 nm. Several parameters affecting the separation and sensitivity of piperacillin were studied, including the pH and concentration of phosphate buffer and concentration of PEO. The linear range of the method for determination of the test drug, in plasma and in peritoneal fluid using cephalothin as an internal standard (IS), was all over the range 0.5-10.0 μg/mL for piperacillin. The application of the proposed method for determination the concentration profiles of piperacillin in plasma and in peritoneal fluid collected after IV injection tazocin for severe intra-abdominal infection patient was demonstrated.