The Role of CD66a Revealed by Its Expression Pattern in Hepatocellular Carcinoma

• Main Authors: Chun-Chieh Wu, 吳俊杰
• Other Authors: Chee-Yin Chai
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/52637843975597579440

碩士 === 高雄醫學大學 === 醫學研究所 === 98 === Background: Hepatocellular carcinoma (HCC) is a primary malignancy of the liver. Most cases of HCC are secondary to either a viral infection (hepatitis B or C) or cirrhosis (alcoholism being the most common cause of hepatic cirrhosis). The patients with HCC usually have a poor prognosis and high mortality. Carcinoembrynic antigen-related cell adhesion molesule 1 (CEACAM; CD66a), formerly known as biliary glycoprotein, is a member of the CEA subfamily and of the immunoglobulin superfamily. The aim of our study was to analyze the expression and possible role of CD66a and CD34 in liver and HCC and its correlation with clinical and pathlogic information. Materials and methods: From 1993 to 2003, paraffin-embedded sections of tissue microarray from 86 patients with HCC and adjacent normal liver tissue are collected in this study. Immunohistochemical analysis was performed, and the expression and the location of CD66a and CD34 were evaluated. We analyze the expression and possible role of CD66a in liver with a viral infection, cirrhosis, or HCC and the relationship between its expression, tumor stage and histologic grade. Results: Among the 86 patients, the CD66a expression in HCC shows diffuse stain, including membrane or cytoplasmic staining in 47 patients. The other 39 patients reveal loss of CD66a expression in HCC. Although the CD66a immunostaining did not correlate with other clinical or prognostic factors of HCC, it did shows significant correlation with the tumor size (P=0.016), fatty change (P=0.009), patients with transcatheter arterial embolization (TAE) (P=0.038) and the microvessel density(P=0.016). Conclusion: The CD66a in normal liver tiisue shows a specific canalicular staining pattern. However, the CD66a expression in HCC shows a variable staining pattern, including membrane stain, cytoplasmic stain , and even loss of expression. In our analysis, the diffuse CD66a stain strongly correlates with small tumor size, fatty change, patients without TAE and more micovessel density. Therefore, the expression of CD66a in tumor cells may invlove the early stage of hepatocarcinogenesis, and the loss expression of CD66a in tumor cells indicates that the tumor cells show high grade transformation.