| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 98 === Abstract
Aristolochia manshuriensis (Guanmuton) (Aristolochiaceae) is a perennial shrub plant, and distributed in the northeastern of the China and Korea. Aristolochia species were used as antibacterial, antiinflammatory, antiasthmatic agents, etc. in the Chinese medicine. In previous reserches, aristolochic acids had been proved to be the most potent constituents of Aristolochia species and were reported to be nephrotoxins and carcinogens with long duration for body metabolism.
The MeOH extract of the stem was partitioned into CH2Cl2 and H2O layers. The CH2Cl2 layer was subjected to Celite column chromatography into n-Hexane, n-Hexane:EA (1:1), EA, MeOH, n-BuOH and H2O-soluble layers. Among them, which was based on the anti-inflammatory assay, the n-Hexane, n-Hexane:EA (1:1), EA and MeOH-soluble layers showed inhibitory effects on superoxide anion generation and elastase release by human neutrophils which induced by fMLP/CB.
In the current phytochemical investigation, the CH2Cl2-soluble layer was subjected to repeated column chromatography. The structures were elucidated by spectroscopic methods that led to the identification of twenty-five compounds, including four aristolochic acids︰ aristolochic acid I (4), aristolochic acid-IIIa (7), aristolochic acid-IVa (6),and aristolochic acid II (5), one sodium salt of aristolochic acids︰sodium aristolochate I (21), five aristolactams ︰aristolatams IIIa (8), aristolactam III (11), aristolactam I (9), aristolactam II (12), and aristolactam Ia (10), three denitroaristolochic acids ︰aristolamide (2), aristolamide II (3), and demethylaristofolin E (16), four aristolochic acid alkyl esters ︰aristolic acid methyl ester (17), 6-methoxyaristolic acid methyl ester (18), aristolochic acid-I methyl ester (13), aristolochic acid-IV methyl ester (14), and aristolochic acid-IVa methyl ester (15), two amides N-trans-feruloylmethoxytyramine (19) and aristopyridinone (25), one benzenoid︰trans-p-Coumaric acid (20), four steroids︰??-sitosteryl-3-O-??-D-glucoside & Stigmasteryl-3-O-??-D-glucoside (22), β-stigmasterol (23), 6β-hydroxystigmast-4-en-3-one (24) and β-sitosterone (25)。
In the anti-inflammatory assay, aristolactam IIIa (8) showed better inhibitory effect on superoxide anion generation and elastase release than genistein, and aristolamide II (3) showed better inhibitory effect on elastase release than genistein.
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