| Summary: | 碩士 === 義守大學 === 生物技術與化學工程研究所碩士班 === 98 === The incidence and mortality of male oral cancer patients ranked at the fourth of the male top ten cancer diseases in Taiwan at 2008. The number of female diagnosed with oral cancer has also increased in these years. The tumors of oral cancer have been proven clinically to be fast-growth, and are easier to metastasize to lung, lymph nodes and other parts. Siegesbeckia orientalis linne and its related plants have been used in China, Taiwan, Korea, Japan and other Asian countries for a long time in anti-inflammation, anti-allergy, cancer adjuvant therapy, and so on. However, very few studies reported on the inhibition effects of S. orientalis linne on the proliferation, migration and invasion of oral cancer cells. In this study, S. orientalis linne was extracted with ethanol (EtOH) and then partitioned by n-hexane, ethyl acetate (EA) and n-butanol. The most active extract was then fractionated by using a chromatographic column. The cytotoxic effects of these extracts and separated fractions on pharyngeal cancer cell line FaDu were examined by MTT assay method. Among the four different extracts, the EtOH extract (IC50 = 60.06 μg/ml for 24 hr treatment) and the hexane extract (IC50 = 57.91 μg/ml for 24 hr treatment) exhibited significant cytotoxicity on FaDu cells. Among the 18 fractions separated from the hexane extract, Fraction no. 11 (IC50 = 13.94 μg/ml for 24 hr treatment) and 12 (IC50 = 20.24 μg/ml for 24 hr treatment) exhibited strong cytotoxicity on FaDu cells. The wound healing assay, cell migration assay and cell invasion assay were used to determine the inhibitory effects of these samples on cell migration and invasion. Among these extracts, the EtOH extract and the hexane extract had significant inhibitory effects on cell migration (IC50 = 94.20 and 44.46 μg/ml, respectively, for 24 hr treatment) and cell invasion (IC50 = 126.85 and 75.00 μg/ml, respectively, for 24 hr treatment). Among the fractions separated from the hexane extract, Fraction 11 and 12 had most significant inhibitory effects on cell migration and cell invasion (the IC50 values were all below 30 μg/ml for 24 hr treatment). Finally, the chemical compositions of these four samples were analyzed by GC-Mass spectrometry. This study has proved that the extract of S. orientalis linne can effectively inhibit the growth, migration and invasion of FaDu pharynx cancer cells. The screening, separation and identification of the bioactive ingredients of S. orientalis linne extracts, as well as the inhibitory effects of these active compounds are now being undertaken in our laboratory.
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