| Summary: | 碩士 === 弘光科技大學 === 食品暨應用生物科技所 === 98 === Cordyceps militaris is also called northern Cordyceps sinensis, an entomopathogenic fungus belonging to the class Ascomycetes. It has similar pharmacological activities to the well known chinese traditional medicine C. sinensis, and has been used extensively as a crude drug and a folk tonic food in East Asia. However, C. militaris is very scarce in nature or time-consuming to get a fruiting body by artificial culture. In this study, Penglai rice was used as a medium for the solid-state cultivation of C. militaris at different temperatures (15, 17, 19 and 21ºC). Temperature-jump were 6, 4, 2 and 0ºC, respectively, with respected to the set ambient temperature (21 ± 1ºC). The humidity and light intensity were controlled throughout the cultivation. Mycelia growth and bioactive components of C. militaris were monitored with and without light. The polysaccharide and cordycepin were analyzed by phenol-sulfuric acid method and HPLC, respectively, in cultivation course with and without light. The most rapid growth of mycelium was found at 15ºC without light. In contrast, the best fruiting body formation was found at 21ºC with light. For the content of bioactive components, results show the highest content of polysaccharide under low temperature cultivated, and they were 240.09 ± 4.0 mg/g (0 day), 273.24 ± 7.0 mg/g (day 10), 293.22 ± 5.2 mg/g (day 20), 313.20 ± 5.6 mg/g (day 30) and 143.87 ± 5.0 mg/g (day 40). For cordycepin contents, the temperature of 21ºC were significantly higher than the other in cultivation with light were 2.50 ± 0.05, 4.84 ± 0.05, 7.16 ± 0.09 and 20.46 ± 0.66 mg/g for 10, 20, 30 and 40 days , respectively.
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