Application of Olfactory Receptor Peptides in Biochip for Gas Sensing
碩士 === 輔仁大學 === 化學系 === 98 === This research focuses on the transmembrane domain biding site of olfactory receptor protein and studies the sequence of olfactory receptor proteins responsible for specific odors. We selected the specific peptide sequence in the third, fourth, and seven transmembran...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2010
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| Online Access: | http://ndltd.ncl.edu.tw/handle/54033647921807557569 |
| Summary: | 碩士 === 輔仁大學 === 化學系 === 98 === This research focuses on the transmembrane domain biding site of olfactory receptor protein and studies the sequence of olfactory receptor proteins responsible for specific odors.
We selected the specific peptide sequence in the third, fourth, and seven transmembrane domains for study. A series of synthetic peptides are prepared with the solid-phase peptide synthesis protocols and microwave method. The PZ crystal will serve as a signal transducer to determine the binding affinity of synthetic peptides and odorants. The synthetic peptides are coating on the surface of a piezoelectric (PZ) electrode for target odorant binding assays. The peptide biochips are screened with 31 different odorants to determine the selectivity of these peptides.
The result showed several selected peptide sequence has high binding affinity with butanoic acid. The specific binding ability between the structure of peptide and odorant molecule are discussed. The result of these studies would be used for further development of biosensor for odorant sensing tools in environmental, food, and medical sample analysis.
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