Production of Trehalose Lipoic Acid Esters Using Immobilized Novozym 435

• Main Authors: Li-Yu,Lai, 賴麗羽
• Other Authors: Shu-Wei,Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/47930033882923639444

碩士 === 大葉大學 === 生物產業科技學系 === 98 === Sugar fatty acid esters are new non-ionic surfactants consisting of a sugar and a fatty acid. Due to their amphiphilic nature, biodegradable, non-toxic and renewable function, they are of great interest in the food, cosmetic and pharmaceutical applications. Currently, esterification reactions are carried out by chemical processes in industries. The biosynthesis of such esters by lipase-catalyzed chemical reactions under mild conditions has become of much current commercial interest. According to currently available reference, most of them used lipase to catalyze the synthesis of sugar fatty acid esters from sucrose, fructose and maltose. Production of trehalose fatty acid with the lipase is quite limited in the literature. The aim of the stady was to establish a process for synthesis of trehalose fatty acid. The synthesis of trehalose fatty acid from trehalose and lipoic, was carried out by a esterification reaction catalyzed by immobilized lipase from Candida Antarctica (Novozym 435). The results showed that we had successfully obtained pure trehalose mono-lipoate which had been confirmed by HPLC-MASS. The actual chemical structure will be identified in the near future.