Studies on the Immobilization of Bromelain by Using Magnetic Chitosan/Fe3O4 Micro/nanoparticles as Carriers

• Main Authors: Shine-Hao Chen, 陳信豪
• Other Authors: Yawo-Kuo Twu
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/30207242496078345752

碩士 === 大葉大學 === 生物產業科技學系 === 98 === In this study, magnetic chitosan/iron (II, III) oxide composite suspension was prepared by combining chitosan with iron ions. Suspension was washed with water until it was neutralized and then bromelain was added to the suspension. The chitosan/iron (II, III) oxide/ bromelain micro/nano particles were produced by low-temperature spray-drying. The larger and smaller particles were collected using an electromagnet collector (EMC) and an electrostatic precipitator (ESP) respectively. Scanning electron microscopy micrographs indicate that the diameters of larger particles collected by EMC were in the range of 500 to 2500 nm while the diameters of smaller particles collected by ESP were in the range of 200 to 1000 nm. Both particles exhibited a rough characteristic. Casein hydrolysis tests were used to determinate the enzyme activities of all the particles. The results indicated that all the micro/nano particles had relative activities higher than 80% and could be reused at least ten times. The relative enzyme activities were increased with increasing chitosan content in the particles. The encapsulation of bromelain with magnetic chitosan/iron (II, III) oxide achieved the goals of enzyme quick recovery and reuse. Key Word: Magnetic chitosan/iron (II, III) oxide/ bromelain micro/nano particles、Low-temperature spray-drying、Bromelain