| Summary: | 碩士 === 朝陽科技大學 === 應用化學系碩士班 === 98 === Zearalenone (ZEA) is one of the mycotoxins which are the secondary metabolites of fungi. It is found in corn, wheat, barley and oats.
In Taiwan, the official analysis method for ZEA is using disposable immunoaffinity column coupling with high performance liquid chromatography (HPLC) which has the detection limit of 10 ppb. However, the modern preservation of grain has reduced the contamination of ZEA in corps. The cost of the specific column and the time consuming are not good for high throughput analysis. If there is a low cost and high throughput screening methods to filter out most uncontaminated samples, a lot of time consuming and cost would be avoided.
Here we report a low cost and high throughput screening method for the analysis of ZEA using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method includes two parts. First, the sample preparation using celite to remove sample matrix is simple and low cost. Second, without general matrix for co-crystal with analyte, aluminum foil is used to obtain low noise analyte spectra in low mass range. The cost of sample preparation for each sample is less than NT$ 10.0 dollars, and analysis time for each sample is only 1 min or less, detection limit can reach to 10 ppb which is comparable with the official method.
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