Evaluate and establish an animal model of thrombosis in the carotid artery of rat induced by laser

• Main Authors: Chin-Hsien Chang, 張晉賢
• Other Authors: Yen-Lin Chang
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/25416533365359106546

博士 === 中原大學 === 醫學工程研究所 === 98 === According to the department of health, out of top ten leading causes of death, stroke has become the second leading cause of death, amounting to 18% of the total mortality rate in Taiwan. Traditional animal models for investigating mechanisms of ischemic stroke mostly take advantages of two different techniques: ligation- and embolism-induced ischemic stroke in rats. Although being widely used, their applications are limited due to technical challenges and low success rate. In order to avoid the technical pitfalls and limitations seen in the animal model, we developed a laser-based microplatform to induce carotid artery thrombosis in rats. Formation of thrombus can be achieved by administrating various amount of photosensizer dyes with simultaneous exposure to appropriated intensity of laser light sources. Coupling the microscopic image analysis which provides the information on vascular thickness of carotid artery, one can formulate critical parameters for desirable experimental outcomes. Once the animal model is developed, We will evaluate therapeutic effects on eliminating carotid artery thrombus induced in experimental animals by administering natto extract, an anti-thrombotic agent extracted from natto, and ferulic acid, an anticancer agent. We developed a real-time laser-based microplatform, equipped with two different laser intensities at wavelength of 532 nm and 650 nm, to induce carotid artery thrombosis in rats. Carotid artery thrombosis was induced by a simultaneous exposure to DPSS Green Laser at 532 nm wavelength with an injection of 60 mg/kg. Rose bengal dye. A significant increase in carotid artery proliferation by 0.08 ±0.02 mm thickness was observed 4 weeks after treatment in treated animals. In contrast, rats treated with various combinations of simultaneous exposures to Laser Diode at 650 nm wavelength with injections of Evans blue dye showed no significant increase in carotid artery proliferation. Furthermore, microscopic analysis of tissue sections taken from rats developed carotid artery thrombosis induced by formal treatment, using H&E staining microscopic and immunofluorescence confocal microscopic analyses, administration of 10ml/kg natto extract and 80 mg/kg ferulic acid to treated rats significantly decreased the proliferation. By altering the various experimental parameters, our long term goal of this investigation is to develop and establish a laser-induced carotid artery thrombosis animal model that closely resembles the underline mechanism of chronic thrombosis seen in stroke patients in clinic, a classic example of bridging both academic engineering and clinical applications in stroke research.