Sensor Development of Induced Force by Optical Tweezers in Bio-Cell Mechanics Application

• Main Authors: Yen-fang Tseng, 曾彥方
• Other Authors: Chih-lang Lin
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/61594526267629685158

碩士 === 中臺科技大學 === 醫學工程暨材料研究所 === 98 === Since 1969, Ashkin announced the methods and the principles of optical tweezers. The optical tweezers technology research and development has gradually matured. Despite, the laser optical tweezers technique has been researched, developed, and used successfully for biological cell manipulation for many years, there are still more improvement in the application of bio-medicine; moreover, the combination between optical tweezers and micro-mechanical sensor of bio-medical research is still rare seen between the countries. The optical tweezers force was affected by laser and optical tweezers system. Therefore, it needs to be calibrated before you measure it. In the research, we measured force relative reference value by every size bead, and proposed a new sensing method, which used the loss of paralysis and active sperm trapping force to measure sperm mobility. Furthermore, proposed an application design combined two-photon absorption photo polymerization (TPP) to make lever biomedical sensors, which is a tool to measure cell stiffness, developing its feasibility of biomedical testing application on combination optical and TPP. In the research, we focus on measured relative of laser power and trapping force plan by every size bead. This research can conduct laser tweezers force basis of any size micro particle element in the sensor. The study showed two main factors effect upon cell communication were the index of cell refraction and absorptive. Furthermore, proposed a new scheme used micro lever to measure cell stiffness, can sieve out and analyze cell stiffness in time. Thence laid the foundation of biological cells interaction research.